Formalin-fixed, paraffin-embedded (FFPE) samples are preserved tissue samples that are used for histological assessment of human or animal tissue. Tissue samples are fixed with formalin to preserve the cytoskeletal and protein structure and then embedded in paraffin so the tissue can be sliced on a microtome. FFPE material is the working horse of pathologists for ascertaining or refining medical diagnoses after general or specific staining procedures, e.g. with antibodies against disease-relevant proteins. For example, fine diagnosis of tumors relies almost entirely on histological assessment of FFPE samples. FFPE samples are available from many patients and from the past, making this the largest potential resource for correlating pathological diagnoses with genomic data, and thereby further refining the power of genomic analyses.
Formalin irreversibly cross-links proteins preserving the structural integrity of the cells. However, the effect of these cross-linking fixatives on the nucleic acids inside cells is harmful, making it challenging to obtain intact genetic information from those samples, as severe degradation and DNA damage occur during sample preparation and long-term storage. In order to use FFPE samples as an efficient source for genomic analysis, a reliable preparation and amplification method is required to generate sufficient quantities of relatively intact genomic DNA for downstream analyses.
TruePrime™ amplification technology is an exceptional tool that is used so far to analyze single cells or purified DNA samples by Next Generation Sequencing (NGS) in a rapid, accurate and cost-effective manner.
Based on the features and benefits of TruePrime™ technology, our R&D team is currently developing a TruePrime™ FFPE DNA Amplification kit that will provide the right tool for the amplification of genomic DNA extracted from FFPE samples.