HIV Reverse Transcriptase
SunScript™ Reverse Transcriptase is a novel revolutionary enzyme that has been engineered from in vitro evolution of human HIV-1 reverse transcriptase for increased thermal stability. It has RNA and DNA-dependent polymerase activity, and is available in two versions: RNaseH+ and RNAseH-.
The main feature of this enzyme is its special ability to perform reverse transcription reactions up to temperatures of 85ºC. This makes SunScript™ Reverse Transcriptase the best choice to efficiently amplify difficult RNA templates, with complex secondary structures, which is a main limitation for conventional reverse transcriptases that are restricted to lower reaction temperatures, making no posible to relax the RNA structure and efficiently perform the complete transcription.
SunScript™ Reverse Transcriptase has also an extreme reaction speed (about 30 bases per second) which makes possible to complete the transcription of very long transcripts in minimum reaction times (5 minutes for a 11 Kb mRNA).
SunScript™ Reverse Transcriptase has other special advantages like the ability to generate high yields of cDNA with an exquisite consistency and reproducibility. It also has an improved sensitivity, being able to perform reverse transcription from starting amounts of 10 pg total RNA.
All these features makes SunScript™ Reverse Transcriptase the optimum enzyme for multiple applications oriented to the study of the transcriptome and gene expresssion analysis. These approaches include reverse transcription combined with end point or quantitative polymerase chain reaction (RT-PCR), synthesis of full lenght cDNA molecules for library construction, cDNA labeling for probes or primer extension analysis.
SunScript™ Reverse Transcriptase is the basis for a new line of Sygnis products based on the special features of this enzyme. Patent protection for SunScript™ Reverse Transcriptase is pending.