Whole Transcriptome Amplification
RNA sequencing (RNA-seq) is rapidly becoming the ideal method for comprehensively characterizing global transcriptome activity. Single-cell transcriptome analysis allows to study the heterogeneity of neighbouring cells in tissues or cell cultures. It has been demonstrated that variability of cells is important for biological function, but cell heterogeneity in terms of the expression profile is also of prime interest for more applied fields like oncology. In order to elucidate the role of cell heterogeneity in health and disease, single-cell transcriptomes can be analysed by new next-generation sequencing (NGS) platforms.
NGS analysis currently requires a few hundred nanograms of RNA. Consequently, accurate whole-transcriptome amplification (WTA) is required to study the transcriptome of a single cell by NGS.
TruePrime™ amplification technology is an outstanding tool that allows Next Generation Sequencing being deployed rapidly, accurately and cost-effectively when analysing single cells or purified DNA samples.
Based on the features and benefits of the TruePrime™ technology, our R&D team is currently developing a TruePrime™ Single Cell WTA kit that will enable the reverse transcription and amplification of limited amounts of RNA to high yields of cDNA.